Application
NEK3 Antibody (K346) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NEK3 antibody detected the NEK3 protein (arrow).Application
Western blot analysis of NEK3 (arrow) using rabbit polyclonal NEK3 Antibody (K346) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NEK3 gene.| Product Name | NEK3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEK3; Serine/threonine-protein kinase Nek3; HSPK 36; Never in mitosis A-related kinase 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NEK3 Serine/threonine-protein kinase Nek3 HSPK 36 Never in mitosis A-related kinase 3 |
|---|---|
| Molecular Weight(MW) | 57705 Da |
| Function | Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin- mediated phosphorylation of PXN and VAV2. Implicated in prolactin- mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2 |
| Tissue Specificity | Up-regulated in malignant versus normal breast tissue. Isoform 2 shows a high level of expression in testis, ovary and brain |
| Cellular Localization | Cytoplasm (By similarity). Cell projection, axon (By similarity) |
Application
NEK3 Antibody (K346) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NEK3 antibody detected the NEK3 protein (arrow).
Application
Western blot analysis of NEK3 (arrow) using rabbit polyclonal NEK3 Antibody (K346) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NEK3 gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
