Application
Western blot analysis of anti-NEK11S/L Pab in mouse kidney tissue lysate. NEK11S/L (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
NEK11S/L Antibody (V336) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the NEK11S/L antibody detected the NEK11S/L protein (arrow).| Product Name | NEK11S/L Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEK11; Serine/threonine-protein kinase Nek11; Never in mitosis A-related kinase 11 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NEK11 Serine/threonine-protein kinase Nek11 Never in mitosis A-related kinase 11 |
|---|---|
| Molecular Weight(MW) | 74192 Da |
| Function | Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation |
| Tissue Specificity | Poorly expressed in cerebellum, trachea, lung, appendix, and uterus |
| Cellular Localization | Nucleus. Nucleus, nucleolus. Note=Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S- arrested cells |
Application
Western blot analysis of anti-NEK11S/L Pab in mouse kidney tissue lysate. NEK11S/L (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
NEK11S/L Antibody (V336) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the NEK11S/L antibody detected the NEK11S/L protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
