Application
Western blot analysis of NEK7 (arrow) using NEK7 Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NEK7 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
NEK7 Antibody (M8) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NEK7 antibody detected the NEK7 protein (arrow).| Product Name | NEK7 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEK7; Serine/threonine-protein kinase Nek7; Never in mitosis A-related kinase 7 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NEK7 Serine/threonine-protein kinase Nek7 Never in mitosis A-related kinase 7 |
|---|---|
| Molecular Weight(MW) | 34551 Da |
| Function | Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1 |
| Tissue Specificity | Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine |
| Cellular Localization | Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, centrosome. Note=Present at centrosome throughout the cell cycle. Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody |
| Entrez Gene | 140609 |
|---|
Application
Western blot analysis of NEK7 (arrow) using NEK7 Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NEK7 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
NEK7 Antibody (M8) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NEK7 antibody detected the NEK7 protein (arrow).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
