Application
The anti-NEK8 Pab is used in Western blot to detect NEK8 in A2058 cell lysate.| Product Name | NEK8 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEK8; JCK; NEK12A; Serine/threonine-protein kinase Nek8; Never in mitosis A-related kinase 8; Nima-related protein kinase 12a |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | JCK NEK12A |
|---|---|
| Alternative Names | NEK8 JCK NEK12A Serine/threonine-protein kinase Nek8 Never in mitosis A-related kinase 8 Nima-related protein kinase 12a |
| Molecular Weight(MW) | 74806 Da |
| Function | Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia (By similarity) |
| Tissue Specificity | Highest expression in thyroid, adrenal gland and skin. Low levels in spleen, colon and uterus. Overexpressed in breast tumors, with highest expression in infiltrating ductal carcinomas and moderate levels in mucinous adenocarcinoma |
| Cellular Localization | Cytoplasm (By similarity). Cytoplasm, cytoskeleton. Cell projection, cilium (By similarity) Note=Predominantly cytoplasmic. Localizes to the proximal region of the primary cilium and is not observed in dividing cells |
| Entrez Gene | 284086 |
|---|
Application
The anti-NEK8 Pab is used in Western blot to detect NEK8 in A2058 cell lysate.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
