Application
NELF Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the NELF antibody detected the NELF protein (arrow).| Product Name | NELF Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NELF; Nasal embryonic luteinizing hormone-releasing hormone factor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NELF |
|---|---|
| Alternative Names | NELF Nasal embryonic luteinizing hormone-releasing hormone factor |
| Molecular Weight(MW) | 60143 Da |
| Function | Couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. Part of the cAMP response element-binding protein (CREB) shut-off signaling pathway. Stimulates outgrowth of olfactory axons and migration of gonadotropin-releasing hormone (GnRH) and luteinizing-hormone-releasing hormone (LHRH) neuronal cells |
| Tissue Specificity | Highly expressed in adult and fetal brain. Weakly expressed in heart, liver, spleen, testis, small intestine, skeletal muscle, peripheral white blood cells and kidney |
| Cellular Localization | Nucleus. Nucleus envelope (By similarity). Nucleus membrane (By similarity). Nucleus matrix (By similarity) Cytoplasm. Cytoplasm, cell cortex (By similarity). Cytoplasm, cytoskeleton (By similarity). Cell membrane; Peripheral membrane protein. Cell projection, dendrite (By similarity). Cell junction, synapse (By similarity). Cell junction, synapse, synaptosome (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Membrane (By similarity) Note=Found on the outside of the luteinizing-hormone-releasing hormone (LHRH) cell membrane and axons projecting from the olfactory pit and epithelium. Associates with transcriptionally active chromatin regions. Detected at the nuclear membranes of CA1 neurons. Cortical cytoskeleton. Localized in proximal apical dendrites. Colocalizes with CABP1 in dendrites and dendritic spines. Myristoylation is a prerequisite for extranuclear localization. Translocates from dendrites to the nucleus during NMDA receptor-dependent long-term potentiation (LTP) induction of synaptic transmission at Schaffer collateral/CA1 synapses of hippocampal primary neurons and in a importin-dependent manner (By similarity) |
| Entrez Gene | 26012 |
|---|---|
| Nucleotide Accession | NP_001124442.1 NP_001124441.1 |
Application
NELF Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the NELF antibody detected the NELF protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
