Application
Immunohistochemistry of NELF in rat brain tissue with NELF antibody at 2.5 μg/ml.Application
Immunofluorescence of NELF in mouse brain tissue with NELF Antibodyat 20 μg/mL.Application
Immunohistochemistry of NELF in mouse brain tissue with NELF Antibodyat 5 μg/mL.Application
Western blot analysis of NELF in Hela cell lysate with NELF antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of NELF in rat brain tissue with NELF antibody at 20 μg/ml.| Product Name | NELF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NELF Antibody: HH9, NELF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor |
| Product description | NELF Antibody: NELF (nasal embryonic luteinizing hormone-releasing hormone factor) is a 530 amino acid transcription factor involved in the migration of LHRH neurons, outgrowth of olfactory axons and suppression of transcription elongation. NELF is found in the peripheral and central nervous system during embryonic development, and is highly expressed in adult testis, kidney and brain. Known to couple NMDA receptor signaling to the nucleus, NELF knockdown impaired GnRH neuronal migration of NLT cells in vitro and the gene encoding NELF has been linked to the development of Idiopathic hypogonadotropic hypogonadism (IHH), a disorder resulting in impaired pubertal maturation and reproductive function.1) Kramer PR and Wray S. Novel gene expressed in nasal region influences outgrowth of olfactory axons and migration of luteinizing hormone-releasing hormone (LHRH) neurons. Genes Dev. 2000; 14:1824-34. |
| Immunogen | Rabbit polyclonal NELF antibody was raised against a 17 amino acid peptide near the center of human NELF. The immunogen is located within amino acids 230 - 280 of NELF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NELF antibody can be used for detection of NELF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NELF |
|---|---|
| Alternative Names | NELF Antibody: HH9 NELF NMDA receptor synaptonuclear signaling and neuronal migration factor Nasal embryonic luteinizing hormone-releasing hormone factor Nasal embryonic LHRH factor |
| Molecular Weight(MW) | Predicted: 58 kDa |
Application
Immunohistochemistry of NELF in rat brain tissue with NELF antibody at 2.5 μg/ml.
Application
Immunofluorescence of NELF in mouse brain tissue with NELF Antibodyat 20 μg/mL.
Application
Immunohistochemistry of NELF in mouse brain tissue with NELF Antibodyat 5 μg/mL.
Application
Western blot analysis of NELF in Hela cell lysate with NELF antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of NELF in rat brain tissue with NELF antibody at 20 μg/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | NELF antibody can be used for detection of NELF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NELF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | NELF Antibody is supplied in PBS containing 0.02% sodium azide. |
