Application
Western blot analysis of Nestin Antibody in K562 cell line lysates (35ug/lane). NEST (arrow) was detected using the purified Pab.Application
Fluorescent confocal image of SY5Y cells stained with AP8742a Nestin antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AP8742a Nestin primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). Nestin immunoreactivity is localized mainly at the cytoskeleton of the SY5Y cells.Application
NEST Antibody flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Nestin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NES; Nestin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFFC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NES Nestin |
|---|---|
| Molecular Weight(MW) | 177439 Da |
| Function | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity) |
| Tissue Specificity | CNS stem cells. |
| Entrez Gene | 10763 |
|---|
Application
Western blot analysis of Nestin Antibody in K562 cell line lysates (35ug/lane). NEST (arrow) was detected using the purified Pab.
Application
Fluorescent confocal image of SY5Y cells stained with AP8742a Nestin antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AP8742a Nestin primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). Nestin immunoreactivity is localized mainly at the cytoskeleton of the SY5Y cells.
Application
NEST Antibody flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
