Application
NEU2 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NEU2 antibody detected the NEU2 protein (arrow).Application
NEU2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NEU2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of NEU2 Antibody (N-term)(Cat#AP12189a) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).Application
NEU2 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NEU2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEU2; Sialidase-2; Cytosolic sialidase; N-acetyl-alpha-neuraminidase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NEU2 Sialidase-2 Cytosolic sialidase N-acetyl-alpha-neuraminidase 2 |
|---|---|
| Molecular Weight(MW) | 42254 Da |
| Function | Hydrolyzes sialylated compounds. |
| Tissue Specificity | Expressed in skeletal muscle, fetal liver and embryonic carcinoma cell line NT2-D1 |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 4759 |
|---|---|
| Nucleotide Accession | NP_005374.2 |
Application
NEU2 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NEU2 antibody detected the NEU2 protein (arrow).
Application
NEU2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NEU2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of NEU2 Antibody (N-term)(Cat#AP12189a) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Application
NEU2 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
