Application
The Neurogenin3 (N-term) polyclonal antibody is used in Western blot to detect Neurogenin3 in mouse liver tissue lysate.Application
Neurogenin3 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the Neurogenin3 antibody detected the Neurogenin3 protein (arrow).Application
Western blot analysis of Neurogenin3 (arrow) using rabbit polyclonal Neurogenin3 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Neurogenin3 gene.Application
Neurogenin3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human large intestine followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Neurogenin3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of Neurogenin3 Antibody (N-term) (Cat#AP2024a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | Neurogenin3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEUROG3; ATOH5; BHLHA7; NGN3; Neurogenin-3; Class A basic helix-loop-helix protein 7; Protein atonal homolog 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ATOH5 BHLHA7 NGN3 |
|---|---|
| Alternative Names | NEUROG3 ATOH5 BHLHA7 NGN3 Neurogenin-3 Class A basic helix-loop-helix protein 7 Protein atonal homolog 5 |
| Molecular Weight(MW) | 23077 Da |
| Function | Acts as a transcriptional regulator. Together with NKX2- 2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types (By similarity) |
| Tissue Specificity | This Neurogenin3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47~76 amino acids from the N-terminal region of human Neurogenin3. |
| Cellular Localization | Nucleus (Potential). |
| Entrez Gene | 50674 |
|---|
Application
The Neurogenin3 (N-term) polyclonal antibody is used in Western blot to detect Neurogenin3 in mouse liver tissue lysate.
Application
Neurogenin3 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the Neurogenin3 antibody detected the Neurogenin3 protein (arrow).
Application
Western blot analysis of Neurogenin3 (arrow) using rabbit polyclonal Neurogenin3 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Neurogenin3 gene.
Application
Neurogenin3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human large intestine followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Neurogenin3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of Neurogenin3 Antibody (N-term) (Cat#AP2024a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
