Application
NFATC1 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the NFATC1 antibody detected the NFATC1 protein (arrow).Application
NFATC1 Antibody (C-term) (AP13900b)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NFATC1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | NFATC1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFATC1; NFAT2; NFATC; Nuclear factor of activated T-cells, cytoplasmic 1; NFAT transcription complex cytosolic component |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NFAT2 NFATC |
|---|---|
| Alternative Names | NFATC1 NFAT2 NFATC Nuclear factor of activated T-cells cytoplasmic 1 NFAT transcription complex cytosolic component |
| Molecular Weight(MW) | 101243 Da |
| Function | Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells |
| Tissue Specificity | Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T- cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T- cells after second antigen exposure |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals The subcellular localization of NFATC plays a key role in the regulation of gene transcription |
| Entrez Gene | 4772 |
|---|---|
| Nucleotide Accession | NP_765977.1 NP_765978.1 NP_765975.1 NP_006153.2 |
Application
NFATC1 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the NFATC1 antibody detected the NFATC1 protein (arrow).
Application
NFATC1 Antibody (C-term) (AP13900b)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NFATC1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
