Application
NFE2 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the NFE2 antibody detected the NFE2 protein (arrow).| Product Name | NFE2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFE2; Transcription factor NF-E2 45 kDa subunit; Leucine zipper protein NF-E2; Nuclear factor, erythroid-derived 2 45 kDa subunit; p45 NF-E2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NFE2 Transcription factor NF-E2 45 kDa subunit Leucine zipper protein NF-E2 Nuclear factor erythroid-derived 2 45 kDa subunit p45 NF-E2 |
|---|---|
| Molecular Weight(MW) | 41473 Da |
| Function | Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron |
| Tissue Specificity | Expressed in hematopoietic cells and also in colon and testis |
| Cellular Localization | Nucleus, PML body. Cytoplasm. Note=The sumoylated form locates to the nuclear bodies PML oncogenic domains (PODs). Translocated to the cytoplasm through interaction with ITCH |
| Entrez Gene | 4778 |
|---|---|
| Nucleotide Accession | NP_001129495.1 NP_006154.1 |
Application
NFE2 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the NFE2 antibody detected the NFE2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
