Application
NFIC Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NFIC antibody detected the NFIC protein (arrow).Application
Fluorescent confocal image of Hela cell stained with NFIC Antibody (Center)(Cat#AP16052c). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIC primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFIC immunoreactivity is localized to nucleus significantly.| Product Name | NFIC Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFIC; NFI; Nuclear factor 1 C-type; CCAAT-box-binding transcription factor; Nuclear factor I/C; TGGCA-binding protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NFI |
|---|---|
| Alternative Names | NFIC NFI Nuclear factor 1 C-type CCAAT-box-binding transcription factor Nuclear factor I/C TGGCA-binding protein |
| Molecular Weight(MW) | 55675 Da |
| Function | Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication |
| Tissue Specificity | This NFIC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179-208 amino acids from the Central region of human NFIC. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 4782 |
|---|---|
| Nucleotide Accession | NP_995315.1 NP_005588.2 |
Application
NFIC Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NFIC antibody detected the NFIC protein (arrow).
Application
Fluorescent confocal image of Hela cell stained with NFIC Antibody (Center)(Cat#AP16052c). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIC primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFIC immunoreactivity is localized to nucleus significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
