Application
Western blot analysis of NFKBIA (Ser32) Antibody in mouse cerebellum tissue lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab.Application
Western blot analysis of Phospho-NFKBIA (Ser32) Antibody in CHO cell line lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab.Application
NFKBIA (Ser32) Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NFKBIA (Ser32) Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
NFKBIA (Ser32) Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of NFKBIA (Ser32) Antibody(Cat#AP7981d) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | NFKBIA (Ser32) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFKBIA; IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; Major histocompatibility complex enhancer-binding protein MAD3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IKBA MAD3 NFKBI |
|---|---|
| Alternative Names | NFKBIA IKBA MAD3 NFKBI NF-kappa-B inhibitor alpha I-kappa-B-alpha Major histocompatibility complex enhancer-binding protein MAD3 |
| Molecular Weight(MW) | 35609 Da |
| Function | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription |
| Tissue Specificity | This NFKBIA (Ser32) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18~48 amino acids from the S32 region of human NFKBIA. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export (By similarity) |
| Entrez Gene | 4792 |
|---|
Application
Western blot analysis of NFKBIA (Ser32) Antibody in mouse cerebellum tissue lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab.
Application
Western blot analysis of Phospho-NFKBIA (Ser32) Antibody in CHO cell line lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab.
Application
NFKBIA (Ser32) Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NFKBIA (Ser32) Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
NFKBIA (Ser32) Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of NFKBIA (Ser32) Antibody(Cat#AP7981d) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
