Application
(LEFT)Western blot analysis of NFU1 Antibody (C-term) in Hela cell line lysates (35ug/lane).NFU1 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of NFU1 Antibody (C-term) in CHO cell line lysates (35ug/lane).NFU1 (arrow) was detected using the purified Pab.Application
NFU1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NFU1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
NFU1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NFU1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HIRIP5 |
|---|---|
| Alternative Names | NFU1 HIRIP5 NFU1 iron-sulfur cluster scaffold homolog mitochondrial HIRA-interacting protein 5 |
| Molecular Weight(MW) | 28463 Da |
| Function | Iron-sulfur cluster scaffold protein which can assemble [4Fe-2S] clusters and deliver them to target proteins |
| Tissue Specificity | Ubiquitous. Expression in adult lung is weak compared to fetal lung |
| Cellular Localization | Mitochondrion. Cytoplasm, cytosol. |
| Entrez Gene | 27247 |
|---|
Application
(LEFT)Western blot analysis of NFU1 Antibody (C-term) in Hela cell line lysates (35ug/lane).NFU1 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of NFU1 Antibody (C-term) in CHO cell line lysates (35ug/lane).NFU1 (arrow) was detected using the purified Pab.
Application
NFU1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NFU1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
NFU1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
