Application
Western blot analysis of Nitro-NFKBIA-nY181 Antibody in HepG2 cell line lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with NFKBIA Antibody (Y181) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | NFKBIA Antibody (Y181) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IKBA, MAD3, NFKBI |
|---|---|
| Alternative Names | NFKBIA IKBA MAD3 NFKBI NF-kappa-B inhibitor alpha I-kappa-B-alpha Major histocompatibility complex enhancer-binding protein MAD3 |
| Molecular Weight(MW) | 35609 Da |
| Function | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription |
| Tissue Specificity | This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164~193 amino acids surrounding Y181 of human NFKBIA. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export (By similarity) |
| Entrez Gene | 4792 |
|---|
Application
Western blot analysis of Nitro-NFKBIA-nY181 Antibody in HepG2 cell line lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with NFKBIA Antibody (Y181) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
