Application
NFYB Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NFYB antibody detected the NFYB protein (arrow).Application
Fluorescent confocal image of Hela cell stained with NFYB Antibody (N-term)(Cat#AP14334a). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFYB primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFYB immunoreactivity is localized to nucleus significantly.| Product Name | NFYB Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HAP3 |
|---|---|
| Alternative Names | NFYB HAP3 Nuclear transcription factor Y subunit beta CAAT box DNA-binding protein subunit B Nuclear transcription factor Y subunit B |
| Molecular Weight(MW) | 22831 Da |
| Function | Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes |
| Tissue Specificity | This NFYB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human NFYB. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 4801 |
|---|---|
| Nucleotide Accession | NP_006157.1 |
Application
NFYB Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NFYB antibody detected the NFYB protein (arrow).
Application
Fluorescent confocal image of Hela cell stained with NFYB Antibody (N-term)(Cat#AP14334a). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFYB primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFYB immunoreactivity is localized to nucleus significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
