Application
Western blot analysis of anti-NIP1 BH3 Domain Pab (Cat.# AP1335a) in HL60 cell lysates (35ug/lane). NIP1 BH3 Domain (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | NIP1 Antibody (BH3 Domain Specific) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NIP1, SEC20L |
|---|---|
| Alternative Names | BNIP1 NIP1 SEC20L Vesicle transport protein SEC20 BCL2/adenovirus E1B 19 kDa protein-interacting protein 1 Transformation-related gene 8 protein |
| Molecular Weight(MW) | 26132 Da |
| Function | SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Required for maintenance of ER network. Implicated in the suppression of cell death. May be involved in mitochondrial autophagy |
| Tissue Specificity | Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine |
| Cellular Localization | Mitochondrion. Endoplasmic reticulum membrane; Single-pass type IV membrane protein |
| Entrez Gene | 662 |
|---|
Application
Western blot analysis of anti-NIP1 BH3 Domain Pab (Cat.# AP1335a) in HL60 cell lysates (35ug/lane). NIP1 BH3 Domain (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
