Application
NIX Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NIX antibody detected the NIX protein (arrow).Application
NIX Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NIX Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of NIX Antibody (Center)(Cat#AP11786c) with A549 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).Application
NIX Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NIX Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BNIP3A, BNIP3H, NIX |
|---|---|
| Alternative Names | BNIP3L BNIP3A BNIP3H NIX BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like Adenovirus E1B19K-binding protein B5 BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A NIP3-like protein X |
| Molecular Weight(MW) | 23930 Da |
| Function | Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor |
| Tissue Specificity | This NIX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-89 amino acids from the Central region of human NIX. |
| Cellular Localization | Nucleus envelope. Endoplasmic reticulum. Mitochondrion outer membrane. Membrane; Single-pass membrane protein (Potential). Note=Colocalizes with SPATA18 at the mitochondrion outer membrane |
| Entrez Gene | 665 |
|---|---|
| Nucleotide Accession | NP_004322.1 |
Application
NIX Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NIX antibody detected the NIX protein (arrow).
Application
NIX Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NIX Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of NIX Antibody (Center)(Cat#AP11786c) with A549 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Application
NIX Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
