Application
NKX1-1 Antibody (Center) western blot analysis in CEM,A549 cell line lysates (35ug/lane).This demonstrates the NKX1-1 antibody detected the NKX1-1 protein (arrow).Application
Fluorescent confocal image of Hela cell stained with NKX1-1 Antibody (Center)(Cat#AP11916c).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NKX1-1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NKX1-1 immunoreactivity is localized to Nucleus significantly.| Product Name | NKX1-1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HPX153 |
|---|---|
| Alternative Names | NKX1-1 HPX153 NK1 transcription factor-related protein 1 Homeobox protein 153 Homeobox protein SAX-2 NKX-1.1 |
| Molecular Weight(MW) | 40659 Da |
| Tissue Specificity | Expressed in hemopoietic progenitor cells. |
| Cellular Localization | Nucleus (By similarity). |
| Nucleotide Accession | XP_931434.4 |
|---|
Application
NKX1-1 Antibody (Center) western blot analysis in CEM,A549 cell line lysates (35ug/lane).This demonstrates the NKX1-1 antibody detected the NKX1-1 protein (arrow).
Application
Fluorescent confocal image of Hela cell stained with NKX1-1 Antibody (Center)(Cat#AP11916c).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NKX1-1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NKX1-1 immunoreactivity is localized to Nucleus significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
