Application
A. Western blot analysis on (A) MCF-7, (B) A375, (C) HeLa and (D) Jurkat cell lysates using anti-Nm23-H1 (NDPK-A) RabMAb (Cat. #AJ1545a), dilution 1:5000.Application
B. Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using anti-Nm23-H1 RabMAb (Cat. #AJ1545a).| Product Name | Nm23-H1 Antibody (NDPK-A) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3036 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NDPKA, NM23 |
|---|---|
| Alternative Names | NME1 NDPKA NM23 Nucleoside diphosphate kinase A Tumor metastatic process-associated protein Metastasis inhibition factor nm23 nm23-H1 Granzyme A-activated DNase |
| Molecular Weight(MW) | 17149 Da |
| Function | Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein- coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination |
| Tissue Specificity | Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA |
| Entrez Gene | 4830 |
|---|
Application
A. Western blot analysis on (A) MCF-7, (B) A375, (C) HeLa and (D) Jurkat cell lysates using anti-Nm23-H1 (NDPK-A) RabMAb (Cat. #AJ1545a), dilution 1:5000.
Application
B. Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using anti-Nm23-H1 RabMAb (Cat. #AJ1545a).| Application Notes | WB:1:1000~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
