Application
Figure 1: Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.Application
Figure 2:Immunofluorescence analysis of Hela cells using NME1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Application
Figure 3: Flow cytometric analysis of Jurkat cells using NME1 mouse mAb (green) and negative control (purple).| Product Name | NME1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4B2 |
| Host Species | Mouse |
| Tested Applications | WBIFFC |
| WB:1:500 - 2000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NDPKA, NM23 |
|---|---|
| Alternative Names | NB AWD NBS GAAD NM23 NDPKA NDPK-A NM23-H1 NME1 |
| Molecular Weight(MW) | 17149 Da |
| Function | Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein- coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination |
| Tissue Specificity | Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA |
| Entrez Gene | 4830 |
|---|---|
| Nucleotide Accession | NP_937818.1 |
Application
Figure 1: Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.
Application
Figure 2:Immunofluorescence analysis of Hela cells using NME1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Application
Figure 3: Flow cytometric analysis of Jurkat cells using NME1 mouse mAb (green) and negative control (purple).| Application Notes | WB:1:500 - 2000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human NME1 expressed in E. Coli. |
