Application
NMB Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the NMB antibody detected the NMB protein (arrow).Application
NMB Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NMB Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of NMB Antibody (Center) (Cat#AP5348c) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | NMB Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NMB Neuromedin-B Neuromedin-B-32 |
|---|---|
| Molecular Weight(MW) | 13252 Da |
| Function | Stimulates smooth muscle contraction in a manner similar to that of bombesin |
| Tissue Specificity | This NMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-53 amino acids from the Central region of human NMB. |
| Cellular Localization | Secreted. |
| Entrez Gene | 4828 |
|---|---|
| Nucleotide Accession | NP_066563.2 |
Application
NMB Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the NMB antibody detected the NMB protein (arrow).
Application
NMB Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NMB Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of NMB Antibody (Center) (Cat#AP5348c) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
