Application
NOD2 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NOD2 antibody detected the NOD2 protein (arrow).Application
NOD2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NOD2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
NOD2 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NOD2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CARD15, IBD1 |
|---|---|
| Alternative Names | NOD2 CARD15 IBD1 Nucleotide-binding oligomerization domain-containing protein 2 Caspase recruitment domain-containing protein 15 Inflammatory bowel disease protein 1 |
| Molecular Weight(MW) | 115283 Da |
| Function | Induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK- gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS) |
| Tissue Specificity | Monocytes-specific. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 64127 |
|---|---|
| Nucleotide Accession | NP_071445.1 |
Application
NOD2 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NOD2 antibody detected the NOD2 protein (arrow).
Application
NOD2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NOD2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
NOD2 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
