Application
A. Western blot analysis on mouse brain tissue lysate using anti-nNos RabMAb (Cat. #AJ1548a), dilution 1:1000Application
B. Immunohistochemical analysis of paraffin-embedded human heart muscle using anti-nNOS RabMAb (Cat. #AJ1548a)| Product Name | nNOS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1855Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NOS1 Nitric oxide synthase brain Constitutive NOS NC-NOS |
|---|---|
| Molecular Weight(MW) | 160970 Da |
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR |
| Tissue Specificity | Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland |
| Cellular Localization | Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine (By similarity). Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity) |
| Entrez Gene | 4842 |
|---|
Application
A. Western blot analysis on mouse brain tissue lysate using anti-nNos RabMAb (Cat. #AJ1548a), dilution 1:1000
Application
B. Immunohistochemical analysis of paraffin-embedded human heart muscle using anti-nNOS RabMAb (Cat. #AJ1548a)| Application Notes | WB:1:500~1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
