Application
Western blot analysis of anti-NOC2L Pab (AP1925a) in mouse liver tissue lysate (35ug/lane). NOC2L(arrow) was detected using the purified Pab (1:60 dilution).| Product Name | NOC2L Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOC2L; NIR; Nucleolar complex protein 2 homolog; NOC2-like protein; Novel INHAT repressor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NIR |
|---|---|
| Alternative Names | NOC2L NIR Nucleolar complex protein 2 homolog NOC2-like protein Novel INHAT repressor |
| Molecular Weight(MW) | 84919 Da |
| Function | Acts as an inhibitor of histone acetyltransferase activity; prevents acetylation of all core histones by the EP300/p300 histone acetyltransferase at p53/TP53-regulated target promoters in a histone deacetylases (HDAC)-independent manner Acts as a transcription corepressor of p53/TP53- and TP63-mediated transactivation of the p21/CDKN1A promoter. Involved in the regulation of p53/TP53-dependent apoptosis. Associates together with TP63 isoform TA*-gamma to the p21/CDKN1A promoter |
| Tissue Specificity | This NOC2L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476~506 amino acids from the central region of human NOC2L. |
| Cellular Localization | Nucleus, nucleoplasm. Nucleus, nucleolus. Note=Translocates from the nucleoli to the nucleoplasm in presence of several stressors like ultraviolet irradiation and actinomycin- D. Predominantly detected in the nucleoli in non-mitotic cells Predominantly detected in nucleoplasma in cells undergoing mitosis |
| Entrez Gene | 26155 |
|---|
Application
Western blot analysis of anti-NOC2L Pab (AP1925a) in mouse liver tissue lysate (35ug/lane). NOC2L(arrow) was detected using the purified Pab (1:60 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
