Application
A. Western blot analysis using anti-NOTCH1 Rabbit Monoclonal on Hek293 cell lysate.(Cat. #AJ1552a), dilution 1:500.Application
B. Immunohistochemical analysis of paraffin-embedded human fetal lungs using anti-NOTCH 1 RabMAb (Cat. #AJ1552a).WB
Notch1 and Hes-1 protein expression were analyzed by Western blot 48 h after miRNA transfection by UTMD.| Product Name | NOTCH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1238Y |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TAN1 |
|---|---|
| Alternative Names | NOTCH1 TAN1 Notch 1 intracellular domain Translocation-associated notch protein TAN-1 |
| Molecular Weight(MW) | 272505 Da |
| Function | Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A (By similarity) |
| Tissue Specificity | In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein (By similarity) |
| Entrez Gene | 4851 |
|---|
Application
A. Western blot analysis using anti-NOTCH1 Rabbit Monoclonal on Hek293 cell lysate.(Cat. #AJ1552a), dilution 1:500.
Application
B. Immunohistochemical analysis of paraffin-embedded human fetal lungs using anti-NOTCH 1 RabMAb (Cat. #AJ1552a).
WB
Notch1 and Hes-1 protein expression were analyzed by Western blot 48 h after miRNA transfection by UTMD.| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
