Application
NOTCH3-Q2306 western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).This demonstrates the NOTCH3 antibody detected the NOTCH3 protein (arrow).Application
Confocal immunofluorescent analysis of NOTCH3 Antibody (C-term)(Cat#AP6220a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | NOTCH3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOTCH3; Neurogenic locus notch homolog protein 3; Notch 3 extracellular truncation; Notch 3 intracellular domain |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NOTCH3 Neurogenic locus notch homolog protein 3 Notch 3 extracellular truncation Notch 3 intracellular domain |
|---|---|
| Molecular Weight(MW) | 243631 Da |
| Function | Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity) |
| Tissue Specificity | Ubiquitously expressed in fetal and adult tissues |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 4854 |
|---|
Application
NOTCH3-Q2306 western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).This demonstrates the NOTCH3 antibody detected the NOTCH3 protein (arrow).
Application
Confocal immunofluorescent analysis of NOTCH3 Antibody (C-term)(Cat#AP6220a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
