Application
NOX4 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NOX4 antibody detected the NOX4 protein (arrow).Application
NOX4 Antibody (N-term) (AP14025a)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NOX4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | NOX4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOX4; RENOX; NADPH oxidase 4; Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RENOX |
|---|---|
| Alternative Names | NOX4 RENOX NADPH oxidase 4 Kidney oxidase-1 Kidney superoxide-producing NADPH oxidase Renal NAD(P)H-oxidase |
| Molecular Weight(MW) | 66932 Da |
| Function | Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity |
| Tissue Specificity | Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein (Probable). Cell junction, focal adhesion (Probable). Nucleus (Probable). Note=May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus |
| Entrez Gene | 50507 |
|---|---|
| Nucleotide Accession | NP_001137308.1 NP_058627.1 |
Application
NOX4 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NOX4 antibody detected the NOX4 protein (arrow).
Application
NOX4 Antibody (N-term) (AP14025a)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NOX4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
