Application
Western blot analysis of NPC2 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). NPC2 (arrow) was detected using the purified Pab.Application
NPC2 Antibody (C-term) (RB18879) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NPC2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
NPC2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NPC2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NPC2; HE1; Epididymal secretory protein E1; Human epididymis-specific protein 1; Niemann-Pick disease type C2 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HE1 |
|---|---|
| Alternative Names | NPC2 HE1 Epididymal secretory protein E1 Human epididymis-specific protein 1 Niemann-Pick disease type C2 protein |
| Molecular Weight(MW) | 16570 Da |
| Function | Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport |
| Tissue Specificity | Epididymis. |
| Cellular Localization | Secreted. Endoplasmic reticulum. Lysosome. |
| Entrez Gene | 10577 |
|---|
Application
Western blot analysis of NPC2 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). NPC2 (arrow) was detected using the purified Pab.
Application
NPC2 Antibody (C-term) (RB18879) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NPC2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
NPC2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
