Application
NPAS2 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the NPAS2 antibody detected the NPAS2 protein (arrow).Application
NPAS2 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the NPAS2 antibody detected the NPAS2 protein (arrow).Application
Fluorescent confocal image of MCF-7 cell stained with NPAS2 Antibody (N-term)(Cat#AP5879a).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NPAS2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NPAS2 immunoreactivity is localized to Nucleus significantly.| Product Name | NPAS2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHE9, MOP4, PASD4 |
|---|---|
| Alternative Names | NPAS2 BHLHE9 MOP4 PASD4 Neuronal PAS domain-containing protein 2 Basic-helix-loop-helix-PAS protein MOP4 Class E basic helix-loop-helix protein 9 Member of PAS protein 4 PAS domain-containing protein 4 |
| Molecular Weight(MW) | 91791 Da |
| Function | BMAL1-NPAS2 heterodimers activate E-box element (5'- CACGTG-3') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins (By similarity) |
| Tissue Specificity | This NPAS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NPAS2. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 4862 |
|---|---|
| Nucleotide Accession | NP_002509.2 |
Application
NPAS2 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the NPAS2 antibody detected the NPAS2 protein (arrow).
Application
NPAS2 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the NPAS2 antibody detected the NPAS2 protein (arrow).
Application
Fluorescent confocal image of MCF-7 cell stained with NPAS2 Antibody (N-term)(Cat#AP5879a).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NPAS2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NPAS2 immunoreactivity is localized to Nucleus significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
