Application
NPL Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the NPL antibody detected the NPL protein (arrow).| Product Name | NPL Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C1orf13 |
|---|---|
| Alternative Names | NPL C1orf13 N-acetylneuraminate lyase N-acetylneuraminate pyruvate-lyase N-acetylneuraminic acid aldolase Sialate lyase Sialate-pyruvate lyase Sialic acid aldolase Sialic acid lyase |
| Molecular Weight(MW) | 35163 Da |
| Function | Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N- glycolylneuraminic acid (Neu5Gc) degradation pathway. Although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded (By similarity) |
| Tissue Specificity | Isoform 2 is expressed in placenta, liver, kidney, pancreas, spleen, thymus, ovary, small intestine and peripheral blood leukocyte |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 80896 |
|---|---|
| Nucleotide Accession | NP_110396.1 |
Application
NPL Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the NPL antibody detected the NPL protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
