Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-NPM (pT199) RabMAb (Cat. #AJ1553b), 1:50,000 dilution. Cells were either (A) untreated (B) treated with CA.Application
B. Immunohistochemical analysis of paraffin-embedded human lymphoma using anti-NPM (pT199) RabMAb (Cat. #AJ1553b).Application
C. Immunofluorescent staining of HeLa cells using anti-Phospho-NPM (pT199) RabMAb (Cat. #AJ1553b).| Product Name | NPM Antibody Phospho (pT199) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T199 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1857Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:50000~200000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NPM |
|---|---|
| Alternative Names | NPM1 NPM Nucleophosmin Nucleolar phosphoprotein B23 Numatrin Nucleolar protein NO38 |
| Molecular Weight(MW) | 32575 Da |
| Function | Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication |
| Tissue Specificity | A phospho-specific peptide corresponding to residues surrounding Theonine 199 was used as an immunogen. This antibody recognizes proteins phosphorylated at T199. |
| Cellular Localization | Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, centrosome. Note=Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis |
| Entrez Gene | 4869 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-NPM (pT199) RabMAb (Cat. #AJ1553b), 1:50,000 dilution. Cells were either (A) untreated (B) treated with CA.
Application
B. Immunohistochemical analysis of paraffin-embedded human lymphoma using anti-NPM (pT199) RabMAb (Cat. #AJ1553b).
Application
C. Immunofluorescent staining of HeLa cells using anti-Phospho-NPM (pT199) RabMAb (Cat. #AJ1553b).| Application Notes | WB:1:50000~200000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
