Application
Western blot analysis of NPM1(arrow) using rabbit polyclonal NPM1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NPM1 gene (Lane 2) (Origene Technologies).Application
Western blot analysis of NPM1 Antibody (N-term) in T47D(lane 1), A2058(lane 2), A375(lane 3) cell line lysates (35ug/lane). NPM1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lymph tissue reacted with NPM1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
NPM1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NPM1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NPM |
|---|---|
| Alternative Names | NPM1 NPM Nucleophosmin Nucleolar phosphoprotein B23 Nucleolar protein NO38 Numatrin |
| Molecular Weight(MW) | 32575 Da |
| Function | Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication |
| Tissue Specificity | This NPM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13~42 amino acids from the C-terminal region of human NPM1. |
| Cellular Localization | Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, centrosome. Note=Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis |
| Entrez Gene | 4869 |
|---|
Application
Western blot analysis of NPM1(arrow) using rabbit polyclonal NPM1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NPM1 gene (Lane 2) (Origene Technologies).
Application
Western blot analysis of NPM1 Antibody (N-term) in T47D(lane 1), A2058(lane 2), A375(lane 3) cell line lysates (35ug/lane). NPM1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lymph tissue reacted with NPM1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
NPM1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
