Application
Western blot analysis of NQO1 Antibody (C-term) in NCI-H460, MCF-7 cell line lysates (35ug/lane). NQO1 (arrow) was detected using the purified Pab.Application
NQO1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NQO1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | NQO1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NQO1; DIA4; NMOR1; NAD(P)H dehydrogenase [quinone] 1; Azoreductase; DT-diaphorase; Menadione reductase; NAD(P)H:quinone oxidoreductase 1; Phylloquinone reductase; Quinone reductase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DIA4 NMOR1 |
|---|---|
| Alternative Names | NQO1 DIA4 NMOR1 NAD(P)H dehydrogenase [quinone] 1 Azoreductase DT-diaphorase Menadione reductase NAD(P)H:quinone oxidoreductase 1 Phylloquinone reductase Quinone reductase 1 |
| Molecular Weight(MW) | 30868 Da |
| Function | The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis |
| Tissue Specificity | This NQO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 245~274 amino acids from the C-terminal region of human NQO1. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 1728 |
|---|
Application
Western blot analysis of NQO1 Antibody (C-term) in NCI-H460, MCF-7 cell line lysates (35ug/lane). NQO1 (arrow) was detected using the purified Pab.
Application
NQO1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NQO1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
