Application
Western blot analysis of NQO1 antibody (Center) in NCI-H460 cell line lysates (35ug/lane). NQO1 (arrow) was detected using the purified Pab.Application
NQO1 antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of NQO1 Antibody (Center)(Cat#AP7350c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | NQO1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NQO1; DIA4; NMOR1; NAD(P)H dehydrogenase [quinone] 1; Azoreductase; DT-diaphorase; Menadione reductase; NAD(P)H:quinone oxidoreductase 1; Phylloquinone reductase; Quinone reductase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIF |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DIA4 NMOR1 |
|---|---|
| Alternative Names | NQO1 DIA4 NMOR1 NAD(P)H dehydrogenase [quinone] 1 Azoreductase DT-diaphorase Menadione reductase NAD(P)H:quinone oxidoreductase 1 Phylloquinone reductase Quinone reductase 1 |
| Molecular Weight(MW) | 30868 Da |
| Function | The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis |
| Tissue Specificity | This NQO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124~153 amino acids from the Center region of human NQO1. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 1728 |
|---|
Application
Western blot analysis of NQO1 antibody (Center) in NCI-H460 cell line lysates (35ug/lane). NQO1 (arrow) was detected using the purified Pab.
Application
NQO1 antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of NQO1 Antibody (Center)(Cat#AP7350c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
