Application
NR0B2 Antibody (Center)(PEI 1:100) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NR0B2 antibody detected the NR0B2 protein (arrow).Application
NR0B2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NR0B2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of NR0B2 Antibody (Center)(Cat#AP11075c) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
NR0B2 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NR0B2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NR0B2; SHP; Nuclear receptor subfamily 0 group B member 2; Orphan nuclear receptor SHP; Small heterodimer partner |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SHP |
|---|---|
| Alternative Names | NR0B2 SHP Nuclear receptor subfamily 0 group B member 2 Orphan nuclear receptor SHP Small heterodimer partner |
| Molecular Weight(MW) | 28058 Da |
| Function | Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box- containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1 |
| Tissue Specificity | Liver. Low levels of expression were detected in heart and pancreas |
| Cellular Localization | Nucleus. Cytoplasm. Note=Colocalizes with NEUROD1 in the nucleus |
| Entrez Gene | 8431 |
|---|---|
| Nucleotide Accession | NP_068804.1 |
Application
NR0B2 Antibody (Center)(PEI 1:100) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NR0B2 antibody detected the NR0B2 protein (arrow).
Application
NR0B2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NR0B2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of NR0B2 Antibody (Center)(Cat#AP11075c) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
NR0B2 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
