Application
Western blot analysis of anti-NRBP Pab in HL-60 cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2). NRBP (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | NRBP Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nrbp1; Madm; Nrbp; Nuclear receptor-binding protein; HLS7-interacting protein kinase; MLF1 adapter molecule |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Madm Nrbp |
|---|---|
| Alternative Names | Nrbp1 Madm Nrbp Nuclear receptor-binding protein HLS7-interacting protein kinase MLF1 adapter molecule |
| Molecular Weight(MW) | 59866 Da |
| Function | May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases (By similarity) |
| Tissue Specificity | This NRBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54~83 amino acids from the N-terminal region of human NRBP. |
| Cellular Localization | Cytoplasm, cell cortex. Endomembrane system. Cell projection, lamellipodium. Note=Colocalizes with activated RAC3 to endomembranes and at the cell periphery in lamellipodia |
| Entrez Gene | 192292 |
|---|
Application
Western blot analysis of anti-NRBP Pab in HL-60 cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2). NRBP (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
