Application
Western blot analysis of anti-NRG1 Antibody (Center) in SK-BR-3 cell line lysates (35ug/lane). NRG1 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of NCI-H460 cells using NRG1 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NRG1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NRG1; GGF; HGL; HRGA; NDF; SMDF; Pro-neuregulin-1, membrane-bound isoform; Neuregulin-1; Acetylcholine receptor-inducing activity; Breast cancer cell differentiation factor p45; Glial growth factor; Heregulin; Neu differentiation factor; Sensory and motor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GGF HGL HRGA NDF SMDF |
|---|---|
| Alternative Names | NRG1 GGF HGL HRGA NDF SMDF Pro-neuregulin-1 membrane-bound isoform Neuregulin-1 Acetylcholine receptor-inducing activity Breast cancer cell differentiation factor p45 Glial growth factor Heregulin Neu differentiation factor Sensory and motor |
| Molecular Weight(MW) | 70392 Da |
| Function | Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development |
| Tissue Specificity | Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific |
| Cellular Localization | Pro-neuregulin-1, membrane-bound isoform: Cell membrane; Single-pass type I membrane protein. Note=Does not seem to be active Isoform 8: Nucleus. Note=May be nuclear. Isoform 10: Membrane; Single-pass type I membrane protein. Note=May possess an internal uncleaved signal sequence |
| Entrez Gene | 3084 |
|---|
Application
Western blot analysis of anti-NRG1 Antibody (Center) in SK-BR-3 cell line lysates (35ug/lane). NRG1 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of NCI-H460 cells using NRG1 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
