Application
Western blot analysis of NSE-Y25 Antibody in mouse cerebellum tissue and Y79, HepG2, Jurkat, CEM cell line lysates (35ug/lane). NSE (arrow) was detected using the purified Pab.Application
NSE Antibody (Y25) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NSE Antibody (Y25) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | NSE Antibody (Y25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ENO2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ENO2 Gamma-enolase 2-phospho-D-glycerate hydro-lyase Enolase 2 Neural enolase Neuron-specific enolase |
|---|---|
| Molecular Weight(MW) | 47269 Da |
| Function | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity) |
| Tissue Specificity | The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons |
| Cellular Localization | Cytoplasm (By similarity). Cell membrane (By similarity). Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form (By similarity) |
Application
Western blot analysis of NSE-Y25 Antibody in mouse cerebellum tissue and Y79, HepG2, Jurkat, CEM cell line lysates (35ug/lane). NSE (arrow) was detected using the purified Pab.
Application
NSE Antibody (Y25) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NSE Antibody (Y25) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
