Application
NSUN2 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the NSUN2 antibody detected the NSUN2 protein (arrow).| Product Name | NSUN2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SAKI, TRM4 |
|---|---|
| Alternative Names | NSUN2 SAKI TRM4 tRNA (cytosine(34)-C(5))-methyltransferase Myc-induced SUN domain-containing protein NOL1/NOP2/Sun domain family member 2 Substrate of AIM1/Aurora kinase B tRNA (cytosine-5-)-methyltransferase tRNA methyltransferase 4 homolog |
| Molecular Weight(MW) | 86471 Da |
| Function | RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5- methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity |
| Tissue Specificity | This NSUN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 426-455 amino acids from the Central region of human NSUN2. |
| Cellular Localization | Nucleus, nucleolus. Cytoplasm, cytoskeleton, spindle. Note=Concentrated in the nucleolus during interphase and translocates to the spindle during mitosis as an RNA-protein complex that includes 18S ribosomal RNA |
| Entrez Gene | 54888 |
|---|---|
| Nucleotide Accession | NP_060225.4 |
Application
NSUN2 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the NSUN2 antibody detected the NSUN2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
