Application
NSUN4 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NSUN4 antibody detected the NSUN4 protein (arrow).Application
NSUN4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NSUN4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
NSUN4 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of NSUN4 Antibody (C-term)(Cat#AP11159b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Product Name | NSUN4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NSUN4 Putative methyltransferase NSUN4 NOL1/NOP2/Sun domain family member 4 |
|---|---|
| Molecular Weight(MW) | 43089 Da |
| Function | Involved in mitochondrial ribosome large subunit biogenesis |
| Tissue Specificity | This NSUN4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the C-terminal region of human NSUN4. |
| Cellular Localization | Mitochondrion. |
| Entrez Gene | 387338 |
|---|---|
| Nucleotide Accession | NP_950245.2 |
Application
NSUN4 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NSUN4 antibody detected the NSUN4 protein (arrow).
Application
NSUN4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NSUN4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
NSUN4 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of NSUN4 Antibody (C-term)(Cat#AP11159b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
