Application
Western blot analysis of GNL3 Antibody (C-term) in HL60 cell line lysates (35ug/lane). GNL3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GNL3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | Nucleostemin (GNL3) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | E2IG3, NS |
|---|---|
| Alternative Names | GNL3 E2IG3 NS Guanine nucleotide-binding protein-like 3 E2-induced gene 3 protein Novel nucleolar protein 47 Nucleolar GTP-binding protein 3 Nucleostemin |
| Molecular Weight(MW) | 61993 Da |
| Function | May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity) |
| Tissue Specificity | Increased levels in lung tissue in cancer patients |
| Cellular Localization | Nucleus (By similarity). Nucleus, nucleolus (By similarity). Note=Shuttles between the nucleus and nucleolus (By similarity) |
| Entrez Gene | 26354 |
|---|
Application
Western blot analysis of GNL3 Antibody (C-term) in HL60 cell line lysates (35ug/lane). GNL3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GNL3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
