Application
NUMBL Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the NUMBL antibody detected the NUMBL protein (arrow).Application
NUMBL Antibody (C-term) (AP14081b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NUMBL Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | NUMBL Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NUMBL Numb-like protein Numb-related protein |
|---|---|
| Molecular Weight(MW) | 64891 Da |
| Function | Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF- kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons |
| Tissue Specificity | This NUMBL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-600 amino acids from the C-terminal region of human NUMBL. |
| Cellular Localization | Cytoplasm (By similarity). Note=Symmetrically distributed throughout the cytoplasm in non dividing neuroblasts of the CNS (By similarity) |
| Entrez Gene | 9253 |
|---|---|
| Nucleotide Accession | NP_004747.1 |
Application
NUMBL Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the NUMBL antibody detected the NUMBL protein (arrow).
Application
NUMBL Antibody (C-term) (AP14081b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NUMBL Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
