Application
NUSAP Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the NUSAP antibody detected the NUSAP protein (arrow).Application
NUSAP antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NUSAP antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | NUSAP Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ANKT |
|---|---|
| Alternative Names | NUSAP1 ANKT Nucleolar and spindle-associated protein 1 |
| Molecular Weight(MW) | 49452 Da |
| Function | Microtubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them |
| Tissue Specificity | This NUSAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the N-terminal region of human NUSAP. |
| Cellular Localization | Cytoplasm (By similarity). Nucleus, nucleolus (By similarity). Cytoplasm, cytoskeleton, spindle (By similarity). Chromosome (By similarity). Note=Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity) Localizes to multiple distinct regions of chromosomes throughout mitosis |
| Entrez Gene | 51203 |
|---|---|
| Nucleotide Accession | NP_057443.2 NP_001123369.1 NP_060924.4 |
Application
NUSAP Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the NUSAP antibody detected the NUSAP protein (arrow).
Application
NUSAP antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NUSAP antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
