Application
The anti-NYREN18 N-term Pab is used in Western blot to detect NYREN18 in mouse liver lysate.Application
Western blot analysis of NUB1 (arrow) using NYREN18 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUB1 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
NYREN18 Antibody (E102) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the NYREN18 antibody detected the NYREN18 protein (arrow).| Product Name | NYREN18 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NYREN18 |
|---|---|
| Alternative Names | NUB1 NYREN18 NEDD8 ultimate buster 1 Negative regulator of ubiquitin-like proteins 1 Renal carcinoma antigen NY-REN-18 |
| Molecular Weight(MW) | 70538 Da |
| Function | Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2 |
| Tissue Specificity | Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2 |
| Cellular Localization | Nucleus. Note=Predominantly nuclear. |
| Entrez Gene | 51667 |
|---|
Application
The anti-NYREN18 N-term Pab is used in Western blot to detect NYREN18 in mouse liver lysate.
Application
Western blot analysis of NUB1 (arrow) using NYREN18 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUB1 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
NYREN18 Antibody (E102) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the NYREN18 antibody detected the NYREN18 protein (arrow).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
