Application
Western blot analysis of Osteocalcin (arrow) using rabbit polyclonal Osteocalcin Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BGLAP gene (Lane 2) (Origene Technologies).Application
Osteocalcin Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the Osteocalcin antibody detected the Osteocalcin protein (arrow).| Product Name | Osteocalcin Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BGLAP; Osteocalcin; Bone Gla protein; Gamma-carboxyglutamic acid-containing protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | BGLAP Osteocalcin Bone Gla protein Gamma-carboxyglutamic acid-containing protein |
|---|---|
| Molecular Weight(MW) | 10963 Da |
| Function | Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium |
| Tissue Specificity | This Osteocalcin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~39 amino acids from the N-terminal region of human osteocalcin. |
| Cellular Localization | Secreted. |
Application
Western blot analysis of Osteocalcin (arrow) using rabbit polyclonal Osteocalcin Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BGLAP gene (Lane 2) (Origene Technologies).
Application
Osteocalcin Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the Osteocalcin antibody detected the Osteocalcin protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
