Application
OXCT1 Antibody (Center) western blot analysis in Hela,HL-60,Jurkat cell line lysates (35ug/lane).This demonstrates the OXCT1 antibody detected the OXCT1 protein (arrow).Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with OXCT1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
OXCT1 Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | OXCT1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OXCT1; OXCT; SCOT; Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial; 3-oxoacid CoA-transferase 1; Somatic-type succinyl-CoA:3-oxoacid CoA-transferase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OXCT SCOT |
|---|---|
| Alternative Names | OXCT1 OXCT SCOT Succinyl-CoA:3-ketoacid coenzyme A transferase 1 mitochondrial 3-oxoacid CoA-transferase 1 Somatic-type succinyl-CoA:3-oxoacid CoA-transferase |
| Molecular Weight(MW) | 56158 Da |
| Function | Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate |
| Tissue Specificity | Abundant in heart, followed in order by kidney, brain, and muscle, whereas in liver it is undetectable; also detectable in leukocytes and fibroblasts |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 5019 |
|---|
Application
OXCT1 Antibody (Center) western blot analysis in Hela,HL-60,Jurkat cell line lysates (35ug/lane).This demonstrates the OXCT1 antibody detected the OXCT1 protein (arrow).
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with OXCT1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
OXCT1 Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
