Application
Western blot analysis of P21Cip1-S130 antibody in Hela cell line lysates (35ug/lane). P21Cip1 (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of P21 Antibody (WAF1) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | P21Cip1-S130 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; p21 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WA |
|---|---|
| Alternative Names | CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1 Cyclin-dependent kinase inhibitor 1 CDK-interacting protein 1 Melanoma differentiation-associated protein 6 p21 |
| Molecular Weight(MW) | 18119 Da |
| Function | May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression Functions in the nuclear localization and assembly of cyclin D- CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex |
| Tissue Specificity | Expressed in all adult tissues, with 5-fold lower levels observed in the brain |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 1026 |
|---|
Application
Western blot analysis of P21Cip1-S130 antibody in Hela cell line lysates (35ug/lane). P21Cip1 (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of P21 Antibody (WAF1) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
