Application
A. Western blot analysis on 293T cell lysates using anti-Phospho-p21 (pS145) RabMAb (Cat. #AJ1568a). Cells were either (A) untreated (B) treated with LP.Application
B. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using Phospho-specific anti- p21 RabMAb (Cat. #AJ1568a).| Product Name | p21 Antibody Phospho (pS145) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1, Cyclin-dependent kinase inhibitor 1, p21;CDK-interacting protein 1;Melanoma differentiation-associated protein 6 |
| Modification | p-S145 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1138 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WA |
|---|---|
| Alternative Names | CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1 Cyclin-dependent kinase inhibitor 1 p21 CDK-interacting protein 1 Melanoma differentiation-associated protein 6 |
| Molecular Weight(MW) | 18119 Da |
| Function | May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression Functions in the nuclear localization and assembly of cyclin D- CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex |
| Tissue Specificity | Expressed in all adult tissues, with 5-fold lower levels observed in the brain |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 1026 |
|---|
Application
A. Western blot analysis on 293T cell lysates using anti-Phospho-p21 (pS145) RabMAb (Cat. #AJ1568a). Cells were either (A) untreated (B) treated with LP.
Application
B. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using Phospho-specific anti- p21 RabMAb (Cat. #AJ1568a).| Application Notes | WB:1:1000~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
