Application
Western blot analysis on (A) NIH/3T3, (B) C6 and (C) Neuro-2a cell lysates using anti-p27/Kip1 RabMAb (Cat. #AJ1569a).Application
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-p27 RabMAb (Cat. #AJ1569a).Application
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using anti-p27 RabMAb (Cat. #AJ1569a).| Product Name | p27/Kip1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cdkn1b, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27;p27Kip1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPFHCR16 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Cdkn1b Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1 |
|---|---|
| Molecular Weight(MW) | 22193 Da |
| Function | Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of cyclin D-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry |
| Tissue Specificity | A recombinant protein in mouse p27/Kip1was used as an immunogen. |
| Cellular Localization | Nucleus. Cytoplasm. Endosome. Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-197, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser- 10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89 (By similarity). Colocalizes at the endosome with SNX6; this leads to lysosomal degradation |
| Entrez Gene | 12576 |
|---|
Application
Western blot analysis on (A) NIH/3T3, (B) C6 and (C) Neuro-2a cell lysates using anti-p27/Kip1 RabMAb (Cat. #AJ1569a).
Application
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-p27 RabMAb (Cat. #AJ1569a).
Application
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using anti-p27 RabMAb (Cat. #AJ1569a).| Application Notes | WB:1:1000~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
